Journal: Oxidative Medicine and Cellular Longevity

Article Title: Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

doi: 10.1155/2022/6776050

Figure Lengend Snippet: IL-37 activation is required for the downregulation of inflammatory cytokines caused by S18. (a, b) HE staining of aortic valve from patients with CAVD or non-CAVD patients, and the expression of IL-37 in valve tissue was detected by immunofluorescent staining. ∗∗ p < 0.01 compared with the non-CAVD group, scale bar = 50 μ m, n = 4. (c, d) Representative immunoblots and the corresponding quantification of IL-37 in HAVICs, n = 5. ∗ p < 0.05 compared with the LPS group. (e, f) Representative immunoblots and ELISA showing the protein expression of ICAM-1 and IL-8 in LPS and S18-treated HAVICs in combination with IL-37 knockdown. Cells with scrambled siRNA were used as controls, n = 4. Data are shown as the mean ± SEM. ∗∗ p < 0.01, and ∗ p < 0.05.

Article Snippet: To determine the role of IL-37 in attenuating the mitochondrial stress in HAVICs, HAVICs were preincubated with recombinant IL-37 (0.1 ng/mL, MCE, HY-P70455) for 1 hour followed by LPS (200 ng/mL) stimulation.

Techniques: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Knockdown

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

doi: 10.1155/2022/6776050

Figure Lengend Snippet: S18 prevents against mitochondrial dysfunction in an IL-37-dependent manner. HAVICs were treated with LPS (200 ng/mL) in the presence or absence of IL-37 (0.1 ng/mL) for 24 hours. (a) Fluorescence images with DCFH-DA dye show the ROS production, n = 4. Scale bar = 100 μ m. (b) Fluorescence images of HAVICs stained with TMRM, n = 4. Scale bar = 50 μ m. Cells were treated with IL-37 siRNA (50 nM, OBiO) following stimulation with LPS (200 ng/mL) with or without S18 (0.5 μ M). (c) Fluorescence images using DCFH-DA fluorescent dye show ROS production, n = 4. Scale bar = 100 μ m. (d) Fluorescence intensity of TMRM staining is shown, n = 4. Scale bar = 50 μ m. Data are shown as the mean ± SEM. # p < 0.05 compared with the control group, ∗ p < 0.05.

Article Snippet: To determine the role of IL-37 in attenuating the mitochondrial stress in HAVICs, HAVICs were preincubated with recombinant IL-37 (0.1 ng/mL, MCE, HY-P70455) for 1 hour followed by LPS (200 ng/mL) stimulation.

Techniques: Fluorescence, Staining, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

doi: 10.1155/2022/6776050

Figure Lengend Snippet: S18 directly binds the monomeric forms of IL-37 (6NCU). The detailed docking mode of (a) heparin/IL-37, (b) S18/IL-37, and (c) GPA/IL-37 with docking pock. The affinity of (d) heparin/IL-37 ( K d = 36.0 μ M), (e) S18/IL-37 ( K d = 84.2 μ M), and GPA/IL-37 ( K d = no binding) binding as analysed by MST assay. Data are shown as the mean ± SEM, n = 3.

Article Snippet: To determine the role of IL-37 in attenuating the mitochondrial stress in HAVICs, HAVICs were preincubated with recombinant IL-37 (0.1 ng/mL, MCE, HY-P70455) for 1 hour followed by LPS (200 ng/mL) stimulation.

Techniques: Binding Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Marine-Derived Piericidin Diglycoside S18 Alleviates Inflammatory Responses in the Aortic Valve via Interaction with Interleukin 37

doi: 10.1155/2022/6776050

Figure Lengend Snippet: Evaluation of the therapeutic efficacy of S18 in IL-37-Tg mice. (a) Timeline diagram of S18 administration in the aortic valve calcification model. (b) Pathological changes in cardiomyocytes and aortic valves in the aortic valve calcification model. HE staining of cardiomyocytes and aortic valves. Scale bar = 200 μ m. (c) Von Kossa staining in the aortic valve calcification model. Scale bar = 50 μ m. (d, e) Representative immunofluorescence images in mouse aortic valves. Scale bar = 50 μ m, n = 5. Data are the mean ± SEM. ## p < 0.01 vs. sham; ∗∗ p < 0.01 vs. the Vit D group.

Article Snippet: To determine the role of IL-37 in attenuating the mitochondrial stress in HAVICs, HAVICs were preincubated with recombinant IL-37 (0.1 ng/mL, MCE, HY-P70455) for 1 hour followed by LPS (200 ng/mL) stimulation.

Techniques: Drug discovery, Staining, Immunofluorescence